Constitutive heterochromatin controls nuclear mechanics, morphology, and integrity through H3K9me3 mediated chromocenter compaction, Manning et al raw data

Figure 1. Heterochromatin histone modification states can be modulated distinctly.Figure 2. Nuclear blebbing and rupture increase upon loss of H3K9me3 while loss of H3K9me2 decreases this effect.Figure 3. Nuclear blebbing and rupture upon loss of H3K9me3 is due to interphase-based behaviors and not dependent on mitotic failures.Figure 4. Nuclear stiffness increases upon loss of H3K9me2 and decreases upon loss of H3K9me3.Figure 5. Chromocenter compaction increases upon loss of H3K9me2, decreases upon loss of H3K9me3, and is unchanged by loss of H3K27me3.Supplemental Figure 1. Phospho-MLC2, lamin A/C, and HP1α levels remain unchanged by inhibitors altering histone modifications.Supplemental Figure 2. H3K9me3 chromocenter heatmaps, DNA compaction, and nucleus characteristics.Supplemental Figure 3. Nuclear blebbing and chromocenter compaction are restored upon dual knockdown of H3K9me2 and H3K27me3.