Effect of other Alexa maleimides on K69C responses.

In these experiments K69C expressing oocytes were first exposed to the test compound (50 µM, 5 minutes), then tested for responses to zinc, pH 6.5 and ATP, then exposed to the challenge compound (50 µM, 5 minutes). The purpose of the challenge compound application was to verify that the test compound had successfully bound to the K69C receptors. If it had not, then responses after the challenge compound would have been similar to the challenge compound given alone. Data for the MTSEA control are also shown in Figure 1 and data for the AM546 control are also shown in Figure 2 and Figure 6B–C.