Anti-DENV scFv effect on DENV infection, dissemination, and transmission rates.

DENV titers in WT, heterozygous and homozygous TADV-A (TADV-AHt and TADV-AHm, respectively), and wMel mosquitoes following a blood meal infected with one of four DENV serotypes are shown. DENV GE from mosquito midguts (at 4 or 14 dpi) and carcasses (14 dpi) of WT, TADV-A, and wMel (for DENV-2 only) mosquitoes were determined using RT-qPCR and calculated using previously published methods. Viral titers in saliva collected from WT, TADV-A, and wMel mosquitoes at 14 dpi were determined using TCID50 on Vero cells.