Illumina RNAseq of ccRCC tumour cell line RCC4

Clear cell renal cell carcinoma (ccRCC) is the most common form of kidney cancer. Here, we used Illumina RNAseq to profile the transcriptomes of a ccRCC cell line RCC4 treated with pro-inflammatory cytokines. Our results here validates the existence of novel isoforms and genes that were discovered in the archival tumour samples by long-read sequencing. In addition, results here revealed potential tumour origin of these novel isoforms and genes. RCC4 cells previously transduced with lentiviral Cas9 expression vector were harvested for total RNA after being treated with pro-inflammatory cytokines IFNg&TNF for 24h . Poly(A)+ RNA molecules were isolated and used as input for sequencing library preparation (NEB Ultra III directional RNA library prep kit) according to manufacturer's instructions. Sequencing libraries were sequenced on an Illumina HiSEq 3000 sequencer.