DataSheet_1_Genome-wide identification of YABBY genes in three Cymbidium species and expression patterns in C. ensifolium (Orchidaceae).docx

Members of the YABBY gene family play significant roles in lamina development in cotyledons, floral organs, and other lateral organs. The Orchidaceae family is one of the largest angiosperm groups. Some YABBYs have been reported in Orchidaceae. However, the function of YABBY genes in Cymbidium is currently unknown. In this study, 24 YABBY genes were identified in Cymbidium ensifolium, C. goeringii, and C. sinense. We analyzed the conserved domains and motifs, the phylogenetic relationships, chromosome distribution, collinear correlation, and cis-elements of these three species. We also analyzed expression patterns of C. ensifolium and C. goeringii. Phylogenetic relationships analysis indicated that 24 YABBY genes were clustered in four groups, INO, CRC/DL, YAB2, and YAB3/FIL. For most YABBY genes, the zinc finger domain was located near the N-terminus and the helix-loop-helix domain (YABBY domain) near the C-terminus. Chromosomal location analysis results suggested that only C. goeringii YABBY has tandem repeat genes. Almost all the YABBY genes displayed corresponding one-to-one relationships in the syntenic relationships analysis. Cis-elements analysis indicated that most elements were clustered in light-responsive elements, followed by MeJA-responsive elements. Expression patterns showed that YAB2 genes have high expression in floral organs. RT-qPCR analysis showed high expression of CeYAB3 in lip, petal, and in the gynostemium. CeCRC and CeYAB2.2 were highly expressed in gynostemium. These findings provide valuable information of YABBY genes in Cymbidium species and the function in Orchidaceae.

YABBY基因家族成员在子叶、花器官及其他侧生器官的叶状发育中扮演着至关重要的角色。兰科（Orchidaceae）家族是最大的被子植物群之一。在兰科中已报道存在某些YABBY基因。然而，YABBY基因在香兰属（Cymbidium）中的功能尚不明确。本研究中，我们在香兰属的剑叶兰（Cymbidium ensifolium）、戈林兰（C. goeringii）和舌唇兰（C. sinense）中鉴定出24个YABBY基因。我们分析了这三个物种保守结构域和基序、系统发育关系、染色体分布、共线性相关性以及顺式元件。此外，我们还分析了剑叶兰和戈林兰的表达模式。系统发育关系分析表明，24个YABBY基因被聚为四个组：INO、CRC/DL、YAB2和YAB3/FIL。对于大多数YABBY基因而言，锌指结构域位于N端附近，而螺旋-环-螺旋结构域（YABBY结构域）位于C端附近。染色体定位分析结果显示，只有戈林兰的YABBY基因存在串联重复基因。在顺式关系分析中，几乎所有YABBY基因都表现出相应的单一定位关系。顺式元件分析表明，大部分元件都集中在光响应元件上，其次是茉莉酸甲酯（MeJA）响应元件。表达模式显示，YAB2基因在花器官中的表达量较高。实时定量PCR分析显示，CeYAB3在唇瓣、花瓣和蕊柱中表达量较高。CeCRC和CeYAB2.2在蕊柱中的表达量也极高。这些发现为香兰属物种中YABBY基因的信息及其在兰科中的功能提供了宝贵资料。