Epigenetic profiling for the molecular classification of metastatic brain tumors. Epigenetic profiling for the molecular classification of metastatic brain tumors

Genome-wide DNA methylation profiling of brain metastases from lung cancer, breast cancer, and melanoma samples. The Illumina Infinium 450K Human DNA methylation Beadchip was used to obtain DNA methylation profiles across approximately 450,000 methylation sites in formalin-fixed paraffin-embedded (FFPE) samples from brain metastases. Samples included 30 breast cancer brain metastases, 18 lung cancer brain metastases, 37 melanoma brain metastases, and 4 samples with brain metastases from patients with uncertain primary. Overall design: Bisulfite converted DNA samples from 89 specimens were analyzed by using the Illumina Infinium 450K Human Methylation BeadChip.