MutMap + in Nipponbare late

We applied MutMap+ method to identify the causal nucleotide changes of rice mutants by whole genome re-sequencing .The mutants was first crossed with Wild type. F1 plant self-pollinated, and the F2 progeny were grown for investigating heading date. We observed segregation between wild type and mutant phenotype in F2 progeny. We next made two bulks of DNA; wild type bulk of 20 individuals from the wild type phenotype and mutant bulk of 20 individuals from the mutant phenotype. The two bulks of DNA were separately sequenced to generate illumina HiSeq 2000 150bp paired end reads.