Depletion of ZNF185 causes delay of keratinocyte differentiation. Homo sapiens

Development of epidermis includes a complicated program of keratinocyte differentiation. Here we study a new membrane LIM-domain containing Zn-finger protein ZNF185 which is expressed in upper layers of human skin and is up-regulated during keratinocyte differentiation in vitro. Interestingly, depletion of ZNF185 causes delay of keratinocyte differentiation with decreased levels of FLG, LOR, LCEs expression. Overall design: To study the role of ZNF185 in keratinocyte differentiation, we performed microarray expression profiling to identify the genes which expression is altered. Human normal epidermal keratinocytes (HEKn) we transiently knocked-down by RNA interference and induced for differentiation for 3 days.