Cochlear fibrocyte and osteoblast lineages expressing type 2 deiodinase identified with a Dio2-CreERt2 allele

Purpose: To define Dio2-expressing cochlear cell types at high resolution in juvenile mice. Methods: Individual fluorescent cells were isolated by micro-manipulator from dissociated pieces of cochlea from heterozygous Dio2ERt2 mice then used to construct single-cell RNA-seq libraries. Results: Consensus clustering of 46 cells revealed two major groups representing fibrocyte, and osteoblast lineages Conclusions: We identify Dio2-expressing fibrocyte and osteoblast lineages Define Dio2-expressing cochlear cell types by high resolution RNA-sequencing of individual fluorescent Dio2-positive cells.