DNase Hypersensitivity Peaks from Rat Schwann Cells

We utilized DNase-Seq to identify open chromatin regions within Schwann cells. This data was used in conjunction with sequence conservation and transcription factor binding site prediction to identify putative cis-regulatory elements critical for Schwann cells. We used three independent biological replicates, and for our studies used sample 'barcode4' as a representative sample for all three. DNase HSS peaks were identified using F-Seq and HOMER, and overlapped to prioritize candidate regions. These regions were then verified using luciferase assays.本研究采用脱氧核糖核酸酶I测序（DNase-Seq）鉴定雪旺细胞（Schwann cells）内的开放染色质区域。将该数据与序列保守性分析及转录因子结合位点预测结果结合使用，以鉴定对雪旺细胞功能至关重要的潜在顺式调控元件（cis-regulatory elements）。本研究设置了三组独立的生物学重复，并选取样本"barcode4"作为三组重复的代表性样本开展后续分析。采用F-Seq与HOMER两款工具识别脱氧核糖核酸酶I高敏位点峰（DNase HSS peaks），通过峰区域重叠对候选调控区域进行优先级排序。随后通过荧光素酶报告基因实验（luciferase assays）对上述候选区域进行功能验证。