Past bacterial communities reconstruction in recent sedimentary deposits, using 16S rRNA gene and transcript

The aim of this study is to evaluate to what extent the comparison of 16S rRNA DNA-based and RNA-based metabarcoding inventories allows discriminating the archived and active bacterial communities in recent lacustrine sediments (i.e. upper 50 cm), and consider the ecological traits of bacterial taxa (i.e., known habitats, trophic modes, energetic metabolism, etc.) in the interpretation of the result of the sorting methods porcess based on the DNA- and RNA-based inventories to better understand their limits.