Single Cell RNA Sequencing Facilitates Quantitative Analysis of CD31+ endothelial cells at day4 of hESC hematopoietic differentiation

Purpose: The goals of this study are to investigate the cell fate desicion and molecular mechanism in CD31+ endothelial cells at the day4 of hematopoietic differentiation. Methods: mRNA profiles of Single CD31+ endothelial cell at day4 of hESC hematopoietic differentiation, were generated by deep sequencing using Illumina GAIIx. The sequence reads that passed quality filters were analyzed with CellRanger pipeline to align reads, generate feature-barcode matrices. Conclusions: We found that the fate of HE and Non-HE has been decided and separated at the emergence of endothelial precursor population during hPSC hematopoiesis. mRNA profiles of Single CD31+ endothelial cell at day4 of hESC hematopoietic differentiation, were generated by deep sequencing using Illumina GAIIx. The sequence reads that passed quality filters were analyzed with CellRanger pipeline to align reads, generate feature-barcode matrices.