Emily Bassett, Stacey Wood, Kevan Salimian, Sandya Ajith, Daniel Foltz, Ben Black (2011) CIL:13608, Homo sapiens, fibroblast. CIL. Dataset

Behavior of a patient-derived human fibroblast line (PD-NC4) containing a mutated chromosome 4 in which the normal centromere is epigenetically silenced and a neo-centromere has formed 25 Mbp down the q arm at a site that lacks centromere repeats. Shown is a z-series of the PD-NC4 chromosome from a metaphase spread stained for CENP-B (red), centromere (ACA, green) and DNA (DAPI blue). See Fig 1D in Bassett et al. 2010. Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors. J Cell Biol 190:177-185.

本研究展示了一株源自患者的皮肤成纤维细胞系（PD-NC4）的行为，该细胞系含有突变型第4号染色体，其中正常的着丝粒通过表观遗传沉默而失活，并形成了一个新的着丝粒，位于q臂的25 Mbp处，该位置缺乏着丝粒重复序列。图中所示为PD-NC4染色体中期的z系列，经CENP-B（红色）、着丝粒（ACA，绿色）和DNA（DAPI蓝色）染色。见Bassett等（2010）图1D。表观遗传学指导的着丝粒特异性可促进aurora B的积累，但不足以有效校正有丝分裂错误。J Cell Biol 190:177-185。