Effect of acetate pre-treatment on macrophages stimulated with S. pneumoniae

The objetive of this study was to evaluate the impact of acetate pre-treatment in alveolar macrophage like cells (MPI) stimulated with Streptococcus pneumoniae. We observed that acetate induce a high modulation of gene expression. Acetate led to similar numbers of up and down-regulated genes and in a enrichment analysis, acetate was shown to regulate different pathways, such as locomotion, cell proliferation and immune processes. Overall design: MPI macrophages cultured in complete medium (RPMI 10% FBS 1% Pen/Strep) were treated with sodium acetate 30mM and after one hour of incubation at 37°C 5% CO2 they were stimulated with heat killed Streptococcus pneumoniae serotype 1, MOI of 30. Cells were then incubated for 18h. Later cells were lysed and RNA was extracted for RNA-Seq