Transcriptomic profiling of ARMCX3 knockdown reveals activation of p53–IFIH1 axis and immune pathways in breast cancer cellsbreast cancer.

ARMCX3 has been implicated in the regulation of mitochondrial dynamics and cancer progression. To investigate its role in breast cancer, we performed RNA-seq following lentiviral-mediated ARMCX3 knockdown in MCF-7 and MDA-MB-231 cell lines, compared to negative controls. The resulting transcriptomic profiles revealed changes in p53 signaling and immune-related pathways, providing insights into the molecular functions of ARMCX3 in breast cancer. Overall design: Breast cancer cell lines MCF-7 and MDA-MB-231 were transduced with lentiviral vectors carrying either ARMCX3-specific shRNA (shARMCX3) or non-targeting control (NC). RNA was extracted from three biological replicates per group (total of 12 samples) and subjected to paired-end 150 bp RNA-seq on an Illumina NovaSeq 6000 platform. Differential expression analysis was conducted to identify ARMCX3-regulated pathways.