RNA sequencing of HECTD3 knockdown during TNFalpha stimulation in primary HUVEC cells

We isolated and validated primary HUVECs from the neonatal umbilical cord vein, and performed genome-wide expression analysis to profile differentially expressed genes in control and HECTD3-KD HUVECs treated with or without TNFa for 2 hours. Compared with control HUVECs, 59 genes showed lower expression in HECTD3-KD HUVECs in response to TNFa. Most of them are NF-?B target genes, including adhesion molecules SELE (E-selectin), ICAM-1, and VCAM-1. Overall design: Examination of differentially expressed genes in control and HECTD3-KD with or without TNFalpha stimulation in primary HUVEC cells.