Transcriptome sequencing of melon fruits at different development stages in different germplasms induced by CPPU

To unravel the regulatory network involved in CuB metabolism in melon fruit in response to CPPU, we conducted transcriptome sequencing at four developmental and ripening stages of both CPPU-treated and control fruit. Employing weighted gene co-expression network analysis (WGCNA) on the transcriptome data, we identified a subset of crucial transcription factors that implicate the CPPU signal in regulating the CuB accumulation pathway.Furthermore, we performed transcriptome analyses on melon fruits treated with CPPU at 3 DAA in introgression lines (ILs) comprising Cmbr mutants and wild type plants. This analysis enabled us to identify the target genes regulated by CPPU-induced CmBr that contribute to CuB accumulation in melon fruits.