The EMT transcription factor ZEB1 governs a fitness-promoting but vulnerable DNA replication stress response - Gene expression profile at single cell level of routinely cultured MDA-MB-231 cells (Sort-Seq)

Cancer cell lines in routine culture are considered as a fairly homogeneous population, but heterogeneity can readily be observed by imunofluorescene microscopy and other single-cell techniques. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of MDA-MB-231 cells in routine culture. Overall design: Routinely cultured MDA-MB-231 cells, plated randomly as single cells into commercially available 384-well capture plates (Sort-Seq Platform, Single Cell Discoveries, Utrecht, NL) by FACS. Empty wells were included as controls.