miRNA expression in high migratory cells

microRNAs are small non-coding RNAs, which promote either mRNAs degradation or block their translation through binding to partially complementary sequences of their target mRNAs. We have previously isolated a subpopulation with accelerated baseline motility (MG cells) or an immotile one (non-MG cells) from a colon cancer cell line (HCT116). In this study, using these subpopulations, we identified miRNAs, which were involved in acquisition of a cell migratory phenotype. Total RNAs were isolated from the MG and non-MG cells, and then miRNAs gene expression was measured by human miRNA microarray (Agilent Technology).