Analysis dataset from: Simultaneous dual-color calcium imaging in freely-behaving mice

Miniaturized fluorescence microscopes (miniscopes) enable imaging of
calcium events from a large population of neurons in freely behaving
animals. Traditionally, miniscopes have only been able to record
from a single fluorescence wavelength. Here, we present a new
open-source dual-channel Miniscope that simultaneously records two
wavelengths in freely behaving animals. To enable simultaneous
acquisition of two fluorescent wavelengths, we incorporated two CMOS
sensors into a single Miniscope. To validate our dual-channel
Miniscope, we imaged hippocampal CA1 region that co-expressed a dynamic
calcium indicator (GCaMP) and a static nuclear signal (tdTomato) while
mice ran on a linear track. Our results suggest that, even when
neurons were registered across days using tdTomato signals, hippocampal
spatial coding changes over time. In conclusion, our novel
dual-channel Miniscope enables imaging of two fluorescence wavelengths
with minimal crosstalk between the two channels, opening the doors to a
multitude of new experimental possibilities.