MIF Values, Calculated Log D Values, and Photophysical Characteristics of Common Water-Soluble Dyes.
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aReactive groups include maleimides (M), azide, biotin, hydrazide, and succinimidyl esters (SE). Where available, dye structures are given in Figure S1.bCalculated using Equation 3. Error values are the propagated error from the standard deviation of three separate measurements each of the experimental and control samples.cFor dyes with hydrolysable reactive groups (i.e. Maleimide or Succinimidyl Esters), logDhyd is the calculated log D using the molecular structure of the dye with a hydrolyzed reactive group. logDunhyd is the calculated log D using the structure of the unhydrolyzed reactive group. For dyes without hydrolysable reactive groups, logDunhyd is the calculated log D. For dyes without a calculated log D value, chemical structures were not available.dε is the extinction coefficient at λmax (ex). QY is the quantum yield. Both are listed as reported by the manufacturers in data sources.eSources of the dye structures in Figure S1 and the photophysical data (λmax (ex), λmax (em), extinction coefficient, and quantum yield) reported in this table, as listed in the references.*Indicates that two separate measurements instead of three were averaged for the experimental samples.**Indicates that two separate measurements instead of three were averaged for the control samples.
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2015-12-02



