Xist-mediate chromatin inaccessibility requires B repeat

The noncoding Xist RNA could mediate chromosome inaccessiblity, especially for the pre-open chromatin regions (enhancer, promoter, CTCF). However, Xist lacking the B-repeats loss the ability of closing the chromatin accessibility. ATAC-seq is consistent with the observation by ATAC-see. XR-PID denotes the Xist RNA polycomb interacting domain, including the entire B-repeats and part of C repeats. Overall design: ATAC-seq for Dox-induced Xist and XistDelXR-PID cells, which contains Xist transgenes in male autosomes.