Gene expression profile at the single cell level of hematopoietic stem/progenitor cells (HSPCs) derived from iPSC at day 12 of differentiation; and from erythroid cultures derived from HS/PC-iPSC at day 4 (RNA-Seq)

Myelodisplastic syndromes are a oligoclonal disease starting at the level of the stem/progenitor compartment. We use scRNAseq to determine the transcriptome changes and clonal evolution of MDS low and high risk iPSC were differentiated for 12 days towards HSPCs using StemDiff Hematopietiese media. Erythroid cultures were derived from these HS/PC-iPSC and scRNAseq performed at day 4 of erythroid differentiation (cultured with SCF, IL3 and EPO)