Potential off-targets from CRIPSR.

"potential_offtargets" tab shows all 158 SNPs that were "fixed" in one genotype compared to the others. Columns are chromosome, position, ref and alternative alleles (based on dm6.54) and the genotype calls for all 9 sequenced lines. The info column is the output from snpEff, the "fixed" column describes which Mtk genotype was different from the others, and the "NearestGenes" column lists the actual gene symbol affected. Rows in yellow were selected for further analysis because they were putatively functional SNPs or in immune-related genes. The next several tabs show the data for individual SNPs. Each tab has a small table with basic SNP annotations and a screen shot from IGV. Most of these show that the alleles are segregating within line even though they were called as ref or alt. The "Mtk SNPs" tab shows the IGV output for the region containing Mtk mutations. The "Predicted_Offtargets" tab shows the regions of the genome that had blast homology to the two gRNAs (at least 14bp match). This tab is standard blastn output except the final column which suggests that very few of these potential off targets had any SNPs.
(XLSX)