Single-cell transcriptional profiles in glomeruli from type 2 db-/- mice

Diabetic nephropathy is a chronic complication of diabetes, presenting albuminuria at an early stage and leading to renal failure. Kidney is a complicated organ, which is responsible for body fluids control, acid-base balance, and removal of toxins. To better understand the progress of diabetic nephropathy, mice renal cortex of control mice, six-week db-/- (increased serum glucose without pathological changes in kidneys), and ten-week db-/- (with pathological changes in kidneys) were collected for single-cell sequencing analyses. A subgroup of glomerular endothelial cells with pro-angiogenetic features was identified in diabetic kidneys. Overall design: Mice renal cortex of control mice (n=2), six-week (n=2) and ten-week db-/- (n=2) were collected for single-cell sequencing analyses (10xGenomics platform).