RNA sequencing analysis of JAG1 overexpressed Ink4a-/- murine astrocytes

Notch signaling is a signaling pathway related to development and homeostasis of tissue. Jagged 1 (JAG1), which is one of the ligand of NOTCH, is highly expressed in tumor and induce proliferation, survival and tumor aggressiveness of cancer cells. This study demonstrate the biological function of JAG1 in tumor progression. To elucidate the role of JAG1, JAG1 was overexpressed in murine Ink4a-/- astrocytes. Our results provide the clue of molecular mechanism in tumor aggressiveness. Overall design: Ink4a-/- murine astrocytes were transfected with pcDNA3.1-puro (Con) or pcDNA3.1-JAG1-puro (JAG1).