Identifying climatic drivers of hybridization with a new ancestral niche reconstruction method

Applications of molecular phylogenetic approaches have uncovered evidence of hybridization across numerous clades of life, yet the environmental factors responsible for driving opportunities for hybridization remain obscure. Verbal models implicating geographic range shifts that brought species together during the Pleistocene have often been invoked, but quantitative tests using paleoclimatic data are needed to validate these models. Here, we produce a phylogeny for Heuchereae, a clade of 15 genera and 83 species in Saxifragaceae, with complete sampling of recognized species, using 277 nuclear loci and nearly complete chloroplast genomes. We then employ an improved framework with a coalescent simulation approach to test and confirm previous hybridization hypotheses and identify one new intergeneric hybridization event. Focusing on the North American distribution of Heuchereae, we introduce and implement a newly developed approach to reconstruct potential past distributions for ancestral..., Published here are the complete Supplements as a collated document, phylogenies, gene trees, alignments, and paleoclimatic interpolations., Standard phylogenetic tree, alignment, and GIS software such as QGIS., # Identifying climatic drivers of hybridization with a new ancestral niche reconstruction method

This repository contains sequence and GIS data associated with the study \"Identifying Climatic Drivers of Hybridization with a New Ancestral Niche Reconstruction Method,\" published in *Systematic Biology*.

## Description of the data and file structure

**projections_ASTRAL_tifs.zip**: A ZIP archive containing ancestral reconstruction projections using the ASTRAL tree, in GEOTiff format. Bio1 interpolated across time periods based on ASTRAL analysis, with date noted in the file name in MYA

**projections_concatenation_tifs.zip**: A ZIP archive containing ancestral reconstruction projections using the concatenation tree, in GEOTiff format. Bio1 interpolated across time periods based on concatenated analysis, with date noted in the file name in MYA.

**trees.zip**: A ZIP archive containing various trees used in the study.

* all_gene_trees_90strip.trees: 277 gene trees in Phylip format
* ...