Stepwise activities of mSWI/SNF family chromatin remodeling complexes direct T cell activation and exhaustion [ATAC-seq]

This study comprehensively profiles the mSWI/SNF complexes throughout acute and chronic T cell stimulation, with and without small molecular inhibitors and degraders. Specifically, we find pan SWI/SNF and complex specific roles in both activation and exhaustion. Overall design: Assay for transposase-accessible chromatin with sequencing of human primary CD8 positive T cells after acute or chronic stimulation using CD3/CD28 dynabeads at 0, 3, 24, 48, 72 hours, 6 days, and 9 days, with and without treatment and of mouse primary CD8 positive T cells after acute or chronic stimulation using CD3/CD28 dynabeads at 0, 48, 72 hours, 6 days, and 9 days, with and without treatment with mSWI/SNF inhibitors.