Comparing gene expression in livers of 1-month vs 6-month old mice following DEN treatment

To look for age-related changes in the liver that take place during DNA damage resolution, we used RNAseq gene expression analysis to characterize mRNA expression profile in livers from 1-month vs. 6-month-old mice either before or 2 days (representing the peak of DNA damage response) and 6 days (representing the resolution phase of DNA damage) after DEN treatment. Overall design: Liver mRNA profile of 1-month and 6-month old C57BL/6 male mice were generated by deep sequencing in triplicate (each sample is a pool of three mice) using Illumina NextSeq. The sequence reads passed quality filters. qRT–PCR validation was performed using TaqMan and SYBR Green assays