Sonostability and the ability of violacein to generate ROS under ultrasound exposure – a sonochemical analysis

his dataset contains complementary experimental data supporting the publication indicated below. The repository hosts raw and processed spectrophotometric data obtained during sonochemical analyses investigating the sonostability of violacein and its ability to generate reactive oxygen species (ROS) under 1 MHz ultrasound irradiation. The dataset has been structured to ensure transparency, reproducibility, and potential reuse in future kinetic or mechanistic studies. File 1 includes spectrophotometric measurements of DPBF degradation under 1 MHz ultrasound exposure, degradation of violacein in the presence of DPBF and ultrasound, and the corresponding kinetic parameter calculations. Each worksheet presents wavelength-specific absorbance values recorded over time, with metadata on experimental conditions (solvent, ultrasound parameters, measurement intervals). Recommended software: LibreOffice Calc or Apache OpenOffice Calc (both open-source alternatives to Excel). File 2 contains spectrophotometric data describing the degradation of NBTC in DMF under 1 MHz ultrasound, and of NBTC in the presence of violacein under the same conditions. The dataset enables direct comparison of violacein’s influence on NBTC degradation kinetics and allows quantitative assessment of singlet oxygen generation efficiency. Recommended software: LibreOffice Calc or Apache OpenOffice Calc. File 3 includes spectrophotometric measurements of SOSG degradation in DMF under 1 MHz ultrasound and in the presence of violacein under identical conditions. The data document fluorescence intensity changes over time and can be used to assess the relative efficiency of ROS generation in violacein-containing systems. Recommended software: LibreOffice Calc or Apache OpenOffice Calc. All files are provided in .xlsx format. The dataset is unique in presenting a systematic, quantitative analysis of the sonostability of violacein and its ability to generate ROS upon ultrasonic activation. It can serve as a valuable benchmark for comparative sonodynamic studies and for the development of new ultrasound-responsive sensitizers. Methods of data acquisition, sample preparation, and detailed experimental conditions are fully described in the related publication listed below.Ziental, D., Czajkowski, M., Majchrzak-Celinska, A., Krajka-Kuzniak, V., Długaszewska, J., & Sobotta, L. (2025). Ultrasound responsive violacein loaded liposomes for sonodynamic therapy. Dyes and Pigments, 113243. https://doi.org/10.1016/j.dyepig.2025.113243