Measurement of NO production by macrophages infected with different Y. pseudotuberculosis strains.
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BMDMs were infected with the indicated strains at an MOI of 10 as described in Figure 1. At 23 h post infection, medium from the infected macrophages were collected and subjected to Griess assay to determine nitrite (NO2−) concentrations. Lipopolysaccharide (LPS)- and IFN γ- treated macrophages are shown as a positive control. Results shown are the means from three independent experiments with duplicate infection wells. Error bars show standard deviations. ***, P
创建时间:
2016-02-23



