RNA sequencing of skin punch biopsies of lesional and non-lesional skin from hidradenitis suppurativa patients

This study investigated the underlying inflammatory pathways and cell types in hidradenitis suppurativa using transcriptomic approaches with RNA sequencing of lesional and non-lesional skin biopsies from hidradenitis suppurativa patients. Three-millimetres punch biopsies were collected from hidradenitis suppurativa lesional skin (edge of an inflammatory nodule) and non-lesional skin (uninvolved skin 10 cm from the active lesion) from 15 patients. The biopsies were obtained from the inguinal or axillary regions. Before the collection of the biopsies, the patients did not receive any systemic or topical treatment for four and two weeks, respectively.