Developing high throughput single-cell single-nucleotide in situ pairwise sequencing for macromolecule spatail-omics

To identified the method which can differentiate in situ SNP modification, we constructed an RNAseq library to identify SNPs in the C6 cell line and parent-specific SNPs in the PVN of C57. Design probes for these SNPs for in situ SNP detection and was applied to detect tumor driver genes mutation and allele specific expression of parental genes. Overall design: Characteristically distributed SNPs in C6 glioma sections, and at the same time obtained parent-specific SNP in situ maps in orthogonal progeny