Data_Sheet_1_Activation and Contraction of Human “Vascular” Smooth Muscle Cells Grown From Circulating Blood Progenitors.docx

Blood outgrowth smooth muscle cells (BO-SMCs) offer the means to study vascular cells without the requirement for surgery providing opportunities for drug discovery, tissue engineering, and personalized medicine. However, little is known about these cells which meant that their therapeutic potential remains unexplored. Our objective was to investigate for the first time the ability of BO-SMCs and vessel-derived smooth muscle cells to sense the thromboxane mimetic U46619 by measuring intracellular calcium elevation and contraction. U46619 (10–6 M) increased cytosolic calcium in BO-SMCs and vascular smooth muscle cells (VSMCs) but not in fibroblasts. Increased calcium signal peaked between 10 and 20 s after U46619 in both smooth muscle cell types. Importantly, U46619 (10–9 to 10–6 M) induced concentration-dependent contractions of both BO-SMCs and VSMCs but not in fibroblasts. In summary, we show that functional responses of BO-SMCs are in line with VSMCs providing critical evidence of their application in biomedical research.

血液外生平滑肌细胞（BO-SMCs）为研究血管细胞提供了无需手术的途径，从而为药物发现、组织工程和个性化医疗提供了机遇。然而，关于这些细胞的知识甚少，这意味着它们的潜在治疗能力尚未得到探索。我们的目标是首次研究BO-SMCs和血管来源的平滑肌细胞感知血栓烷类似物U46619的能力，通过测量细胞内钙离子升高和收缩。U46619（10^-6 M）增加了BO-SMCs和血管平滑肌细胞（VSMCs）的细胞质钙离子，但并未影响成纤维细胞。在U46619作用后10至20秒内，钙信号达到峰值，且在两种平滑肌细胞类型中均如此。值得注意的是，U46619（10^-9至10^-6 M）诱导了BO-SMCs和VSMCs的浓度依赖性收缩，但在成纤维细胞中并未观察到这种现象。总之，我们证明了BO-SMCs的功能性反应与VSMCs一致，这为它们在生物医学研究中的应用提供了关键证据。