Effects of GSC overexpression on migration of HCC.

Hep3B-GSC cells were infected by GSC lentivirus and were selected with puromycin. (A) Wound-healing assay shows that Hep3B-GSC cells migrated faster than normal Hep3B or Hep3B cells without treatment after 24 h and 48 h. The cleared area of Hep3B-GSC was less than the controls. (50× original magnification, size bar: 100 µm) Results were analyzed with the Student t test. (B) Giemsa staining indicated the number of Hep3B-GSC cells that migrated through Matrigel was higher than Hep3B cells without treatment or treated with negative control (100× original magnification, size bar: 100 µm). The data are presented as the mean ± SD of at least three independent experiments. Results were analyzed using Student's t test.