Transcriptional Analysis of Type II Alveolar Epithelial Cells in the Presence or Absence of Foxp3+ Regulatory T Cells during Experimental Acute Lung Injury Resolution

These studies profiled the expression of mRNA in lung type II alveolar epithelial cells during lipopolysacchride induced lung injury in the presence or absence of Foxp3+ Regulatory T Cells Baseline, uninjured lung type II alveolar epithelial (AT2) cells or resolving AT2 cells, in the presence or absence of Foxp3+ regulatory T cells (Tregs), were sorted (7 days after intratracheal instillation of LPS for resolving conditions) and mRNAs differentially expressed (DE) between control AT2 cells, resolving AT2 cells with Tregs present, or resolving AT2 cells in mice depleted of Tregs were identified using microarrays. Control lung type II alveolar epithelial (AT2) cells or two sets of AT2 cells obtained during a time point of lung injury resolution either in the presence or absence of regulatory T cells were sorted for mRNA analysis. mRNA expression was profiled using Mouse Gene 2.1 ST arrays (Affymetrix, Santa Clara, CA) which cover over 28,000 RefSeq transcripts