Single cell gene expression, immune receptor and cell surface marker expression profiling of Spike-specfic splenic T and B cells of immunized mice

Spike-specific T and B cells from splenocytes of immunized mice were profiled with 10x sequencing for gene expression (Chromium Next Automated GEM 5' v2 kit), cell surface marker (Chromium Automated 5' Feature Barcode kit) and immune receptor (Chromium Automated Mouse BCR/TCR Amplification and Library Construction kit) Overall design: Splenocytes from immunized mice were FACS sorted with fluorescently-labelled Spike protein tetramer or Spike pMHC tetramer for antigen specific T and B cells for 10x single cell profiling Please note that the processed data files generated from both *GEX and *CITE samples are linked to the corresponding *GEX sample records.