Intracellular ROS formation was measured using flow cytometry in yeasts loaded with H2DCFDA. Samples were treated with APCP for 2 minutes and analyzed at 30, 60 and 120 seconds after treatment. Data a
Sample multiplexing in flow cytometry is a powerful technique which allows for reduction of error, inclusion of control samples for batch effect correction, and reduction in both time and consumable u
(A) The result of flow cytometry and (B) the statistical analysis. Data (± SD) are representative of three independent experiments. Student’s t test was performed to determine statistical significance
