Metabarcoding data for diatom samples in "Recommendations for the preservation of environmental samples in diatom metabarcoding studies"

Fastq files obtained by metabarcoding of 216 samples from " Baricevic, Chardon et al., 2022. Recommendations for the preservation of environmental samples in diatom metabarcoding studies. MBMG". Metabarcoding was done as follow: A 312 bp fragment of the rbcL chloroplastic gene was amplified from DNA extracts using Takara LA Taq® polymerase and an equimolar mix of the forward primers Diat_rbcL_708F_1, 708F_2, 708F_3 and the reverse primers R3_1, R3_2 (Vasselon et al. 2017a), following the protocol of Chardon et al. (2020). For each DNA sample, a single step PCR amplification was performed in triplicate in a final volume of 25 μl. After validating PCR amplification, 19 µl of each PCR product per sample were pooled together and 50 µl of this pool were transferred into an individual well of a 96-well microplate. The resulting three plates with 216 samples were sent to “GenoToul Genomics and Transcriptomics” (GeT-PlaGe, Auzeville, France) where library preparation, final library pool and the sequencing with Illumina MiSeq System with paired-end sequencing kit (V2, 250 bp × 2) were performed.