Activation of a Hippocampal CREB-pCREB-miRNA-MEF2 Axis Modulates Individual Variation of Spatial Learning and Memory Capability

To identify novel miRNAs involved in the regulation of learning and memory formation, we used the Morris water maze task to distinguish inbred wild-type C57BL/6J mice with good or poor learning and memory capability. C57BL/6J mice which undergo water maze task were sacrificed immediately after training. The hippocampus was harvested for miRNAs expression analysis with miRNA array (Phalanx Mouse & Rat miRNA OneArray 2.0, annotation based on: miRBase release 15). These differentially expressed miRNAs were applied for further investigation. The hippocampus in experiment mice underwent Morris water maze with good performances or poor performances were harvested immediately after training (n=4 in each groups, total 2(Replicates)*2(groups)=4 arrays were used).