The WDR5-H3K4me3 epigenetic axis regulates OPN expression to compensate PD-L1 function to promote pancreatic cancer immune escape [ChIP-seq]

Total RNA was isolated from normal C57BL/6 mouse pancreas, PANC02-H7, and UN-KC-6141 tumors from tumor-bearing mice. The RNA was used for RNA-Seq. The gene expression profiles between normal mouse pancreas and orthotopic pancreatic tumors were compared, and differentially expressed genes were identified. Normal mouse pancreas and tumors from tumor-bearing mice were used for chromatin fragment preparation. Anti-H3K4me3 antibody was used for ChIP, followed by library construction and ChIP-sequencing. The genome-wide H3K4me3 profiles between normal mouse pancreas and orthotopic pancreatic tumors were compared. Genes with differential H3K4me3 depositions at their promoter regions were identified.