Secretin targets interstitial cells of Cajal to regulate intestinal motility

Secretin plays an important role in gastrointestinal (GI) physiology by stimulating bicarbonate and bile secretion to neutralize acidic chyme and aid in lipid absorption. Secretin also slows intestinal motility, an effect attributed to vagal afferent pathways. In this study, we show evidence for a novel function of secretin involving a non-neural mechanism mediated by interstitial cells of Cajal (ICC). Secretin receptors (SCTR) were found to be abundantly expressed by ICC in the deep muscular plexus (ICC-DMP) of the small intestine. Secretin reduced small intestinal contractions in the presence of tetrodotoxin (TTX) and suppressed cholinergic transmission. Ca2+ imaging demonstrated that the inhibitory effects of secretin occur through inhibition of Ca2+ transients in ICC-DMP, an effect previously linked to inhibition of intestinal contractions. SCTRs are linked to function via Gas, secretin increased cAMP levels in ICC-DMP, and responses were inhibited by a selective PKA antagonist. Our..., , # Secretin targets interstitial cells of Cajal to regulate intestinal motility

Dataset DOI: [10.5061/dryad.v6wwpzh6q](10.5061/dryad.v6wwpzh6q)

## Description of the data and file structure

All experimental data associated files collected for the paper are organized below: 

### Files and variables

#### File: README.docx

**Description:** Copy of file list as a Word table

#### File: STMAPS.zip

**Description:** The folder contains all data associated with calcium imaging experiments. 

**Sub-folders:**

###### *STMAPS_3Hz_EFS:*

COLOR_11-6-23_40x_gc6_IM_nicard200nM_EFS3hzcontinuous_20secCNTRL_sct100nM_5min_STMapCell1-1000frames

COLOR_11-6-23_40x_gc6_IM_nicard200nM_EFS3hzcontinuous_20secCNTRL_STMapcell1-1000frames

###### *STMAPS_10Hz_EFS:*

81424_40x_gc6kit_dmp_mrs25001uM_lnna100uM_2xstim10sec_1min10sec_10hz10sec_secondBest_STMap1-1after copy

81424_40x_gc6kit_dmp_mrs25001uM_lnna100uM_2xstim10sec_1min10sec_10hz10sec_secondBest_STMap1-1before copy

81424_40x_gc6kit_dmp_mrs25001uM_ln...,