miRNA gene expression signatures for IhhN protein induced cells

Previous studies demonstrated that hererozygous missense mutations in IHH could lead to a decreasing IHH signaling efficacy and cause brachydactyly type A1 (BDA1). For revealing difference of Gli1-mediated downstream regulatory effects between wild type IHH signaling and E95K mutant IHH signaling, we have employed whole genome microarray miRNA expression profiling as a discovery platform to identify miRNAs with the potential to take part in Gli1-mediated cis-regulatory network. miRNA expressions of Wild type IhhN protein-induced or E95K mutant IhhN protein-induced were measured after 48-hour incubation in the presence of 750nM protein. Non-protein induced cells were as control.