Structure Guided Discovery of Ancestral CRISPR-Cas13 Ribonucleases

NIAID Data Ecosystem2026-05-02 收录

CRISPR-Cas13功能验证

核酸酶进化分析

数据链接：

https://www.ncbi.nlm.nih.gov/sra/SRP516111 数据链接链接失效反馈

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资源简介：

Sequencing results for small RNA-sequencing assays, mismatch tolerance assays, and target flanking sequence (also known as protospacer flanking sequence) assays.

创建时间：

2024-06-27

Next Generation Sequencing Facilitates Transcriptomes Quantitative Analysis of HEK293T cells transfected with the plasmids respectively encoding RfxCas13d, crRNA and NeuN.. Next Generation Sequencing Facilitates Transcriptomes Quantitative Analysis of HEK293T cells transfected with the plasmids respectively encoding RfxCas13d, crRNA and NeuN.

CRISPR-Cas13功能验证

差异表达转录组分析

To determine what effect the collateral activity of RfxCas13d has on HEK293T cells upon targeting overexpressed NeuN. The plasmids encoding RfxCas13d, NeuN and NT/targeting crRNA into HEK293T cells. A

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Next Generation Sequencing Facilitates Transcriptomes Quantitative Analysis of HEK293T cells transfected with the plasmids respectively encoding RfxCas13d, crRNA and NeuN.

CRISPR-Cas13功能验证

差异表达转录组分析

NIAID Data Ecosystem20

Intrinsic RNA targeting constrains the utility of CRISPR-Cas13 systems [RNA-seq]. Intrinsic RNA targeting constrains the utility of CRISPR-Cas13 systems [RNA-seq]

CRISPR-Cas13功能验证

RNA靶向编辑技术

CRISPR-Cas13 systems have been adapted as versatile toolkits for RNA-related applications. Here we systematically evaluate the performance of several prominent Cas13 family effectors (Cas13a, Cas13b a

NIAID Data Ecosystem10

LbuCas13a directly targets DNA and elicits strong trans-cleavage activity

CRISPR-Cas13功能验证

基因编辑工具靶向分析

Image data can efficiently support scientific verification, cross-platform reuse, and subsequent AI analysis

Figshare2025-06-21 更新20

Intrinsic RNA targeting constrains the utility of CRISPR-Cas13 systems [RIP-seq]

CRISPR-Cas13功能验证

慢病毒载体

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