Influence of AhR genotype on the memory/effector phenotype of the precursor CD8+ cells (a) and upon the Th0 cytokine profile (b).

Naïve CD8+ cells from AhR+/− or AhR−/− mice (n = 3) were isolated and the frequency of naïve, central memory, effector memory and effector cells was characterised by flow cytometry (a). Cells were cultured under Th0 conditions for 5 days. Cells were then stimulated with PdBU and ionomycin in the presence of brefeldin A. The polarised cells were then permeabilised and stained using fluorescent anti-IL-17A (PE [Y-axis]) and -IFN-γ (APC [X-axis]) labelled antibodies and appropriate isotype control labelled antibodies (b). In each case, cells (25000) were analysed by flow cytometry.Influence of AhR genotype on the memory/effector phenotype of the precursor CD8+ cells (a) and upon the Th0 cytokine profile (b).