Data underlying the publication_Evaluating the efficiency of enhanced coagulation for nanoplastics removal using flow cytometry

The aim of this study was to investigate the removal of nanoplastics from tap water using an enhanced coagulation approach and to evaluate the process efficiency with fluorescence-based flow cytometry (FCM). Fluorescently labelled polystyrene beads (PS-OSO₃⁻) of varying sizes were used to assess the impact of particle size on removal efficiency under different coagulant (Fe³⁺) doses. In addition to fluorescence-based analysis, turbidity measurements—widely used as a standard for water quality and recently adopted for nanoplastics removal—were conducted and compared with FCM. Enhanced coagulation was achieved by optimizing mixing conditions, specifically by varying the slow mixing speed at 100, 50, and 25 revolutions per minute (rpm).

本研究旨在通过强化混凝方法，探究自来水中纳米塑料的去除效果，并采用荧光流式细胞术（fluorescence-based flow cytometry, FCM）评估该工艺的处理效率。实验选用不同粒径的荧光标记聚苯乙烯微球（PS-OSO₃⁻），以考察不同混凝剂（Fe³⁺）投加量下，粒径对纳米塑料去除效率的影响。除荧光分析外，研究还开展了浊度测定实验——浊度作为水质检测的通用标准，近期亦被应用于纳米塑料去除效果的评估——并将其结果与荧光流式细胞术的检测数据进行对比。本研究通过优化搅拌条件实现强化混凝，具体通过将慢速搅拌转速设置为100、50与25转每分钟（rpm）完成。