Study on the changes of intestinal microbiota imbalance and oral immunity induced by antibiotics in SD rats

To investigate the changes of intestinal microbiota structure and oral immune function in dysregulated intestinal microbiota. Design: Twenty-four SD rats were selected. After the same caries model was established in all rats, different concentrations of ceftriaxone sodium solution were injected to establish different degrees of intestinal flora imbalance model, and the control group was not injected. The rats were divided into healthy control group (N=8), mild intestinal disorder group (N=8) and moderate intestinal disorder group (N=8). The intestinal microbiota structure of SD rats with intestinal disorder was identified by high-throughput sequencing technology, and the concentration of SIgA in oral samples was determined by ELISA. Results: The decrease of oral immune factors and the changes of intestinal microbiota structure were determined when intestinal microbiota was moderately dysregulated. The species richness and diversity of intestinal microbiota in mild/moderate imbalance group were significantly lower than those in control group. The salivary SIgA concentration in moderate imbalance group was significantly lower than that in mild imbalance group and control group. Conclusion: Moderate intestinal microbiota dysregulation in SD rats indirectly leads to decreased salivary SIgA concentration and increased host oral disease susceptibility. The results of this study suggest that there is a certain correlation between oral immune function and intestinal inflammation related to intestinal microbiota dysregulation, and intestinal microbiota dysregulation should not be ignored when studying the pathogenesis of systemic and chronic diseases.