Genomic profiles of oxidative stress-induced renal cell carcinomas developed in wild-type and BRCA1+/- SD rats

Fenton reaction-based repeated oxidative stress via ferric nitrilotriacetate (Fe-NTA) causes renal cell carcinoma (RCC) with genetic alterations similar to those in humans. We conducted high-resolution microarray comparative genomic hybridization with Fe-NTA induced RCC obtained from both wild-type and BRCA1+/- rats to evaluate the involvement of BRCA1 haploinsufficiency in carcinogenesis. The carcinogenesis protocol was started with 20 wildtype and 19 BRCA1+/- male Jcl: SD rats. 17 of each genptype of rats developed a renal cell carcinoma in total. Genomes of 8 samples from RCCs for each genotype (16 in total) were profiled on Agilent 180K rat genome CGH microarrays.