Systemic infection rates of SYNV antigenomic cDNA and derivatives in Nicotiana benthamiana supported by various combinations of core proteins and viral suppressors of RNA silencing (VSRs).

Equal volumes of Agrobacterium cultures carrying the plasmids encoding each of the antigenome derivatives, core proteins and VSRs were mixed and infiltrated into N. benthamiana leaves. Systemically infected plants were confirmed by symptom inspection and RT-PCR. The number of systemically infected per inoculated plants in three independent experiments (Exp.) were used to calculate the total percentage of systemic infectivity.a SYNV antigenomic cDNA and its derivatives were cloned into the pCB301 binary vector. MR-sc4-RFP is an SYNV antigenome minireplicon derivative containing an sc4 gene substitution for the N ORF and a RFP gene substitution for the P ORF.b N: pGD-N; P: pGD-P; L: pGD-L; NPL: pGD-NPL containing N, P and L tandem expression cassettes.c VSRs: pGD vectors expressing BSMV γb, TBSV p19 and TEV P1/HC-Pro, respectively.Systemic infection rates of SYNV antigenomic cDNA and derivatives in Nicotiana benthamiana supported by various combinations of core proteins and viral suppressors of RNA silencing (VSRs).