SILAC experiment of HEK 293 cells upon RPS26 silencing

In recent years, there is emerging pool of evidence suggesting that ribosomal composition plays a role in selection of transcripts that are actively translated at given time and tissue. Some ribosomal subunits are not the core components of 40S ribosome and alternatively can be assembled or disassembled from 40S subunit. For example, a 40S ribosomal protein - RPS26 is one of such alternative ribosomal subunits. In ordered to investigate what proteins are under the RPS26-depended translation, we performed stable isotope labeling using amino acids in cell culture (SILAC) experiment 48 hours post siRNA-based RPS26 silencing in HEK293 cells and revealed the pool of proteins that are up or downregulated upon RPS26 depletion.